You analyzed the C18 content of a sample of ground beef. You took 5 g of beef, homogenized into buffer and centrifuged. The volume of the supernatant was 14 ml.
You took 2 ml, of the supernatant, extracted the fatty acids and then made the fatty acid methyl ester (FAME) derivatives.
The final volume of the FAME sample was 50 µl. 3 µl of this were injected onto the GC. The area of the C18 peak was 14753.
2µl of a 0.01 mg/ml C18 standard were injected in a second run. The area of the C18 peak was 18334.
What was the concentration of C18 in the original tissue (in µg/g of beef)