1. Propose an experimental approach to determine whether a protein that you are studying has quaternary structure.
2. Make your case for which type of intermolecular interaction is the most important for protein folding (there is no single correct answer).
3. Draw a 2D free energy landscape for a protein with 3 energetically equivalent native states.
4. One of the roles of molecular chaperones is to bind to the nascent protein chain during protein synthesis. Why is this often necessary?5. Why are x-rays used to generate images of proteins?
6. X-ray crystallography, NMR, and Cryo Electron Microscopy are the three major methods of atomic resolution structure determination. What are the advantages and disadvantages of these approaches to structure determination.