Q1. Give and explain in detail 10 mistakes in the steps of spread plate method that can affect the results of the method. Elaborate further for each mistake.
Q2. Based on answers in no 1, explain in detail 10 ways to correct the mistakes mentioned. Elaborate further for each wake to improve the results.
Q3. Explain in detail 5 reasons why we need to incubate all plates in an inverted position for 24 hours at 37 C. Elaborate further.
Q4. Explain briefly serial dilution method.
Q5. Ethyl alcohol is used in spread plate method. Explain in detail the use and function in this procedure.
(Place the L shaped rod into the beaker and add a sufficient amount of ethyl alcohol)
Q6. Explain in detail why soil sample is used as a sample in serial dilution method. Elaborate further.
Q7. Soil sample is used as a sample in serial dilution method. How does it related to the techniques for isolation of pure culture-spread plate method? Explain in detail.
Q8. Explain in detail the function of sterile saline in serial dilution procedure. Elaborate further.
Q9. Spread plate inoculation procedure is for the separation of cells of a mixed culture or soil sample. Elaborate further and justify.
Q10. Give conclusion for the techniques for isolation of pure culture-spread plate method. Explain in detail.
Q11. It is found that the plate turns cloudy so, the colony on the plate is invisible and cannot be seen and counted. Give and explain 5 possible reasons why and suggest 5 ways how to overcome this problem. Elaborate further and justify.
Q12. It appears that the colony appear to be clumped and cannot be counted. Give and explain 5 possible reasons why and suggest 5 ways how to overcome this problem. Elaborate further and justify.
Q13. Explain in detail why after complete, we need to immerse the rod in alcohol and reflame.