Problem:
I'm a molecular bio novice, and I'm trying to understand the differences between regular PCR and RACE PCR.
If it wanted to sequence my PCR product that might be hundreds, if not thousands of bps long, I should use RACE PCR correct?
My goal is to sequence the active site of some enzymes I'm interested in, but I'm not sure how to go about designing PCR primers for a specific site on my protein. Additionally, the active site is over 100 amino acids long, which would imply that my PCR product has to be over 300 bps long.
Regular PCR amplicons have trouble amplifying long strands of DNA correct?
I was thnking about using RACE PCR to amplify my entire cDNA strand that is 4000 bps long and then just sequencing the RACE product.
Question: Would RACE work for that many bps in my cDNA?