Assignment:
I did a lab on column chromatography and thin layer chromatography, and our sample was a spinach extract.
I am trying to figure out if there is a correlation between the partition coefficient and the Rf used in thin layer chromatography. Is there one?
For the column chromatography we used a 9:1 hexane: acetone, thenn 1:1 hexane and acetone, then used methanol. We got three fractions and ideally the colors were supposed to be yellow for the 9:1, green for 1:1: and finally light green for methanol. Instead we got light yellow, light green, even lighter green. Why did that happen?
When I did the thin layer chromatography, only the spinach extract and the 9:1 actually moved up the plate. Nothing happened for the 1:1 and the methanol. I did repeatedly place the sample on the spot, but I don't understand what went wrong?
Provide complete and step by step solution for the question and show calculations and use formulas.