Problem:
In pretty much every transformation protocol I've seen SOC medium is used to grow the bacteria for a short while after the transformation and before plating.
I've usually substituted LB medium for this step for easy retransformations, but I gather that the SOC medium is supposed to make some difference in efficiency, or else the simpler LB medium would be recommended in the protocols.
Question: Why is SOC medium used for transformations?
Question: What difference does substituting with LB medium cause for the transformation efficiency?
Provide better results for the following question.