Discussion Post: DNA Sequences
• Which of the double-stranded DNA sequences below will require more heat to separate the two strands (select Option I or Option II)?
• Why is more heat is required to separate the strands?
Option I
5'-GGACTCCCGGGTGAG-3'
3'-CCTGAGGGCCCACTC-5'
Option II
5'-AACTGTTTAAAGACA-3'
3'-TTGACAAATTTCTGT-5'
• What will be the mRNA sequence that is produced during the transcription of the DNA sequence below? Hint: As we discussed in class, there is a slight difference in the nucleotides used in DNA vs RNA.
5'-ACGTCAGGTTTCCGT-3'
• Based on what was discussed in Lecture 9, name four enzymes/proteins that play a role in human DNA replication, and briefly describe the specific role each plays during human DNA replication.
The response should include a reference list. One-inch margins, Using Times New Roman 12 pnt font, double-space and APA style of writing and citations.