1. Why is it neccessary to digest a plasmid with individualrestriciton enzymes first, then follow with a combination ofdigestions?
2, How is the number of fragments produced associated with the numberof cut-sites available to a restriction enzyme?
3. How could one change in a DNA nucleotide alter the formation ofthe translated protein?
4. Why don't the negatively charged phosphate groups keep DNA strands apart?
5. What is the name for the process in the breakdown of glucose to pyruvate.