Why is 595nm used as the wavelength to measure the, Biology

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Why is 595nm used as the wavelength to measure the

1. Why is 595nm used as the wavelength to measure the absorbance of Coomassie Blue? What does the wavelength tell us about Coomassie blue, or any other substance?

2. The pKa for R group of glutamic acid is 4.3 (The pKa values of amino group and carboxyl group are 9 and 2.5 each. At pH 2, is this amino acid posivitely charged or negatively charged and why?

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