Why immunostaining compared to a focus assay, Biology

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Why immunostaining compared to a focus assay

Problem:

Question 1: Why might the titre of most animal viruses be different when using an immunostaining compared to a focus assay? Which assay would suggest a higher titre?

Question 2: Would you expect the effects of stimulus frequency to be qualitatively the same in cardiac muscle from different species inlcuding humans.

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