A aliquot which is having 25.0 mL of an aqueous quinine solution was diluted to 50.0 mL and found to have an absorbance of the 0.656 at 348 nm when calculated in a 2.50-cm cell. A second 25.0 mL aliquot was mixed with a 10.00 mL of a solution, which is having the 25.7 ppm of quinine; after dilution to 50.0 mL, this solution.