Which carbon atoms of glucose are lost in pyruvate

Question

Problem:

Question 1: Which carbon atoms of glucose are lost in the first pass of pyruvate into and through the TCA cycle?

Question 2: Why does the loss of an allele during genetic drift affect small populations more significantly than large populations?

1. Small populations have fewer alleles, so each plays a proportionally greater role.
2. Small populations have a greater percentage of fit individuals.
3. Small populations cannot adapt because natural selection requires a minimum population size to work.
4. Small populations have more gene flow.
5. Small populations have greater diversity.

Give reasoning with your answer.