Differential and Biochemical Tests
Post-Lab Questions:
1. Which bacterial plates contained catalase positive bacteria?
2. Which plates contained catalase negative bacteria?
3. Did the colonies from each plate show the same reaction? Why or why not? How can you interpret this?
4. Were any controls used in this experiment?
5. What reaction would you expect when performing a negative control in the catalase assay?
6. What reaction would you expect when performing a positive control in the catalase assay? What would it mean if a known catalase positive bacteria did not produce bubbles after the addition of H2O2?
7. If flavoprotein performs the alternate pathway of transferring an electron directly to oxygen rather than to the next component of the ETC, what toxic molecules are produced? What other effects of bypassing the ETC have on the cell?