What would be effect on results of a column chromatogram


What would be the effect on the results of a column chromatogram if

a. the top layer of sand was not included in making the column?
b. solvent was not maintained above the adsorbent, and a crack developed in the column?
c. you do not number the test tubes in which you are collecting fractions?

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Chemistry: What would be effect on results of a column chromatogram
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