Problem:
I've used HPLC (high performance liquid chromatography) before (once, so I'm barely even qualified to know what it stands for) so I was surprised when my lab mate told me she would be using an alternate technique to isolate a protein.
Question: What exactly are differences in HPLC and FPLC (fast protein liquid chromatography) instruments?
Question: Why would FPLC be a better technique than HPLC to use with proteins?
Question: What situations make HPLC preferable?
Please explain the difference , provide better techniques and define the situations that make HPLC preferable.