Assignment task: You perform a viable plate count assay using the selective environmental conditions best to selectively grown and differentiate E. coli to try and determine which food is contaminated and the source of the outbreak.
1. What type of oxygen environment and temperature would be best to incubate your plates to selectively grow and detect E. coli?
2. What media would be best used to selectively grow and detect E. coli in your second experiment? Provide a brief explanation as to how this media works and what results you would expect for E. coli?
3. Addition of which antibiotic would make your new selective/differential media even more selective for E. coli?