Problem 1: What is the ug/ul concentration of the tube 100 protein standard?
Problem 2: Explain in detail how you set up the serial dilution series of your clarified serum sample. Be sure to include all steps taken to prepare the complete serial dilution series. Do not copy/paste! In your own words.
Problem 3: You have obtained absorbances for both the duplicated set of BSA standard dilutions AND the duplicated set of your serially diluted unknown protein sample.
Next you create a standard curve.
Explain how you will use your standard curve data to determine the concentration of your unknown clarified serum sample.
Problem 4: What is the sample volume in the Coomassie Assay?