Problem:
Question: While in the lab, you and your lab partner are using the same type of microscope with the only difference being that you are using a source of illumination of ? = 300 nm and your partner is using a source of illumination of ? = 425 nm.
Which of you is getting the better resolution? Explain briefly.
Question 1: What is the theoretical resolution for your microscope if you use the 40X lens and a wavelength of ? = 475 nm (Show calculation and report result in ?m):
Question 2: Using the resolution value you calculated in part b, can you see objects that are 0.0002 mm apart as being distinct? Explain briefly.
Question 3: If you are looking at a specimen with the 10X objective and your partner is using the using the 4X objective, will there be a difference in resolution if you are both using a source of illumination of ? = 525 nm? Explain briefly and show calculations.
Question 4: You are looking at a specimen under the microscope using the 4x objective lens and have it in perfect focus but not quite centered. You want to see some more detail in your specimen so you increase the magnification and change to the 10x objective lens. To your surprise your specimen has disappeared! What has happened and how can you correct it?
show your all calculations.