1) Assayed for LDH activity were 5μL of a sample that wasdiluted 6 to 1. The activity in the reaction vessel, which has avolume of 3ml, is 0.30U. what is the ΔA/min observed? what is the relative activity of the original sample?
2) Of the original "undiluted" sample from the question above,10μL of a 5 to 1 dilution were used to measure protein concentration. With the use of a standard curve, this is found tobe 140μL. What is the specific activity of the original sample?