What is the reason for the use of oil in imagining, Biology

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What is the reason for the use of oil in imagining

Problem

Light microscopes are generally used for magnification up to 400X. For higher magnification, such as 1000X, oil immersion is used between the specimen and the objective lens. What is the reason for the use of oil in imagining? What is a hemacytometer and how is it used to determine number of cells?

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