Problem:
Usually DNA sample manipulation is realized with an ice box at hand in order to avoid degradation, and also its storage is set at -20ºC. Nevertheless, DNA has been obtained from really ancient samples that were not in a cold environment and even our DNA is at 36ºC. Furthermore, when performing PCR, samples are put into a massive heat shock and at high temperatures.
Required:
Question 1: How needed are those protocols in order to grant correct DNA quality?
Question 2: How much can a sample be left without need to worry?
Question 3: How does this degradation happen (DNAse free environment)
Does anybody know any source of this kind?