What is the formula to calculate cfu/ml in original specimen


Problem:

A sample was diluted by placing a 0.1 mL aliquot into 0.9 mL of diluent,and 100mL of this dilution was pour-plated. After incubation, 217 colonies appeared. What is the CFU/mL in the original specimen?

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Biology: What is the formula to calculate cfu/ml in original specimen
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