This is a scientific report. Do the introduction, Aims of practical, methods and reference parts. Questions and discussion parts. Top of that they gave some articles about the disease please read those articles as well. do your best.
Practical report
- Read the article entitled "Pernicious anaemia" (1997) by Toh, B.H., van Driel, I.R. and Gleeson, P.A., New England Journal of Medicine, 337, p1441-1448
- Obtain an entire set of results for all serum samples for the Western blotting and immuno-peroxidase staining from the other students at your bench. Summarise the results in a table.
- Determine which of the patients can be diagnosed with pernicious anaemia.
Avoid the temptation to cut and paste anything from the manual, journal articles or web pages. It is really obvious and easily detected by anti-plagiarism software.
Your Prac reports should include the following:
- Title and author: A title should reflect the report content. Below the title include your name and practical partner's name and practical session
- A short paragraph that describes the Aim/Aims of the Prac
- An Introduction that gives a background to the experiments. What is pernicious anaemia? Provide some background to the experimental procedure and what you hope to achieve. You can make reference to published articles here. This should be half to one page in length.
- A Methods section that summarises the main steps you followed with reference to the detail in the Prac manual. Do not include materials lists. You can use sub- headings for different techniques. Write in paragraph form, not dot-points. It should not look anything like the manual. The manual is full of unnecessary details. Make sure your methods reflect modifications made to procedures. Don't copy straight from the manual. If you used 200µL instead of 100µL of serum your methods should state the correct figure.
- The Results section should systematically describe your results in detail, with reference to Figures and Tables that display your data. All data presented in Figures or Tables, including diagrams, should also be commented on in the text of the Results section. Provide Figure Legends to accompany your Figures and a heading for Tables. You can use subheadings in this section if you like.
- In the Discussion you should discuss the significance of your findings. Explain what your Results mean and how they contribute to understanding pernicious anaemia. Mention if you achieved your Aims and if there were any factors that adversely contributed to the experiment. If you had technical problems and you did not obtain good results explain what occurred, give a scientific explanation for the failed result. Suggest how the problem might be remedied. Could you improve the experiment? Provide references to support your argument.
- In your Prac write-up, include the answers to the following questions in whichever section you think appropriate eg Introduction, Materials and Methods, Discussion etc:
Questions
a. What is the expected size of the protein being detected by the sera from pernicious anaemia patients?
b. Which subunit of the proton pump does this correspond to?
c. Describe another method that could be used to detect and quantitate the levels of anti-proton pump antibodies in patient's sera?
d. The secondary antibody that was used in the Western and immuno-histochemistry was a sheep anti-human Ig-HRP conjugate. What is the antigen that this antibody is specific for? What animal was this antibody raised in? What is the enzyme that is conjugated to the antibody?
- Refer to published papers (primary literature) as well as secondary literature in your Prac report and use Endnote to reference your write up
- Obtain electronic images of your mouse stomach diagrams from your phone or the Library scanner
- Ensure all figures and tables are labelled properly. Can they be interpreted without reading the text? Use legends where necessary.
- Word limit 2000-2500