Experiment: Effect of Temperature on Enzyme Activity
Pre-Lab Questions
1. What reaction is being used to study the effect of temperature on enzyme activity in this experiment? Temperature can affect the enzyme catalytic reaction.
2. What does an increase in balloon diameter mean with respect to enzyme activity? The activity increases as well
3. Why is important to know this information before starting Experiment #1? The diameter of the balloon will show the activity of the enzyme based upon temperature.
Post-Lab Questions
1. What is the enzyme in this experiment? The catalase. What is the substrate? The Hydrogen Peroxide
2. What is the independent variable in this experiment? Temperature. What is the dependent variable? Balloon diameter
3. How did temperature affect enzyme function? Hot temperature causes high enzyme activity, the cold has less activity, but the room temperature's activity was slower to react.
4. Is there a negative control in this experiment? No negative controls. To revise we would use water, therefore nothing would be expected to happen.