Problem:
In a 100µl reaction containing 0.1 pmol of a Michaelis enzyme with Km = 2.5 x 10-6 M and saturating substrate concentration, product was formed at an initial velocity of 5.0 x 10-5 M min-1 .
Required:
Question 1: To what concentration must the substrate be reduced in order to achieve half this velocity?
Question 2: If the [S] from part a is increased by 50%, by what percent will the initial velocity be increased?
Question 3: If the [S] is kept at the value from part a, but the enzyme concentration is increased by 50%, by what percent will the initial velocity be increased?
Question 4: What is kcat for this enzyme?
Question 5: What is the efficiency of this enzyme?
Question 6: Would this enzyme be considered a perfect enzyme? Explain.
Show the pedigree and your calculation.