Problem
I. The S549N CFTR allele results in a CFTR protein that is made and moves to the cell surface, however, the channel gate does not open properly. Explain how this mutation would affect the movement of chloride ions and water molecules into and out of the cell.
II. An error in DNA replication results in a codon for glutamic acid being changed to a codon for alanine This change occurred in an exon of a gene. With only this information, would you expect this mutation is likely to affect the final protein? Reference specific characteristics of these two amino acids in your answer.
III. What is the complementary DNA strand to the DNA sequence below:
5' - ATGAAAGGGATATA - 3'
IV. -The following RNA strand was produced:
5' UAC AUG AGU GAA 3'
Which DNA strands could have been the template for this RNA?
V. A biologist isolates a large, unidentified molecule and determines that it appears to be used for long-term energy storage. Which class of biological macromolecule might she have isolated? If it could possibly be more than one, what characteristics could she test for to determine which is more likely? Explain your answer.