What evidence contributed to conclusions concerning nature, Biology

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What evidence contributed to conclusions concerning nature

Problem

I. Briefly describe the plasmid maintenance assay used to 11nd replication origins in yeast.

II. Are cereuiside and pombe origins similar or different? How? What evidence contributed to the conclusions concerning the nature of pombe origins. Which (or either/neither] seem more like metazoan origins"?

III. What is the ORG? How do the "AT hooks" in ORG-4 help explain how pombe origin selection might work?

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