Problem:
Question 1: Answer the question:
a) What DNA component is found at the 3' end of the DNA strand?
b) What DNA component is found at the 5' end of the DNA strand?
c) DNA replication is always carried out from _______ to ______ end?
d) DNA is read by a polymerase from _______ to ______ end?
e) How many H-bonds are formed between guanine and cytosine.
Question 2: Many enzymes and proteins become denatured upon exposure to high temperatures. If cells replicate DNA at body temperature (37°C), why is PCR conducted at temperatures 50-95°C?
Question 3: What does RNA stand for? What are the differences between DNA and RNA? (4 points)
Question 4: Does every gene code for a protein? Why?
Question 5: Primers are one of key ingredients for polymerase chain reaction (PCR). How do living cells fulfill this requirement?
Please describe the answer of all the questions.