Assignment task: Gene Cloning
Q1. What is the definition for gene cloning?
Q2. What is the difference between the terms gene cloning and horizontal gene transfer? What do these terms refer to?
Q3. What are restriction enzymes? What are some of their properties and what role do they play in gene cloning?
Q4. What is a plasmid and what purpose does it play in gene cloning? What are some of the essential elements of a plasmid and what role do they play?
Q5. How is plasmid DNA different than the chromosomal DNA in bacteria?
Q6. Describe the role of PCR and DNA ligase in gene cloning.
Q7. What does the term transformation mean? How is it done and what is a challenge of transformation?
Q8. How could you tell if you have successfully transformed a plasmid?
Q9. Using GFP or another case, explain the process (important steps) for transferring a gene from one organism to another.