Discussion:
Understanding enzyme kinetics - Henri-Michaelis-Menten model
An enzyme with a Km of 0.36 mM was assayed using substrate concentrations of 3 x 10E-7 M, 9.5 x 10E-5 M, 1.5 x 10E-4, 1.16x10E-3, 3 x 10E-3 M, 0.01 M, 0.08 M. At a substrate concentration of 0.08 M, the initial velocity was 192 nM/min. Calculate the initial velocity at each of the other substrate concentrations. Graph the calculated velocities using the Henri-Michaelis-Menten model.