A solution buffered at pH 6.5 contains 0.5 mM Histidine, 1.0 mM Arginine, 0.1 mM lysine, and 0.7 mM Tyrosine was loaded onto an anion-exchange column and completely washed with a pH 6.5 buffer. Which amino acid came off the column first? A) Arg B) Lys C) Tyr D) His E) None, all would be retained on the column at pH 6.5