Types of chromatography- Purification of organic compounds
Depending on the nature of the stationary and the mobile phases, the different types of chromatographic methods generally used are in a given table,
Table
Type of Chromatography
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Mobile/Stationary Phase
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Uses
|
|
Column chromatography or adsorption
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Liquid/Solid
|
Large scale separations
|
|
Thin-layer chromatography
|
Liquid/Solid
|
Qualitative analysis (identification and characterization of organic compounds)
|
|
High performance liquid chromatography
|
Liquid/Solid
|
Qualitative and quantitative analysis
|
|
Gas-liquid chromatography (GLC)
|
Gas/Liquid
|
Qualitative and quantitative analysis
|
|
Partition chromatography or ascending paper chromatography
|
Liquid/Liquid
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Qualitative and quantitative analysis of polar organic compounds (sugars, α-amino acids and inorganic compounds)
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It is constant for a specific substance (component) within a given set of conditions. Hence, it is possible to identify the several components by determining their Rf values.