The kinetics of an enzyme is measured as a function of substrate concentration in the presence and absence of 2 mM inhibitor. The data from the experiments are shown below:
a). What are the apparent Vmax and Km in the absence and in presence of the inhibitor?
Vo versus [S] plot:
without inhibitor
Vmax = 0.0629
Km=-3.269
with inhibitor
Vmax= 0.1486
Km= -3.098
Lineweaver-Burk plot
without inhibitor
Vmax=44.64
Km= -4.464
with inhibitor
Vmax=45.045
Km=-1.501
b). What kind of inhibition is it?
c). What is the KI of the inhibitor?