The Gram stain procedure sounds really simple. However, there are several factors that can affect the results if is not done correctly. Here are some examples we can think about. For example, do you think it is possible to overheat smears? If yes, how can it affect Gram stain results? Do you think that the concentrations of the reagents can affect Gram stain results? If yes, how? What are your thoughts about excessive decolorizing, excessive rinsing and excessive counter-staining? Explain.