5.00-mL solution aliquots contain PCP were carefully measured into five separate 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of PCP (2.000 µg/mL) were added into each flask: 0.000, 0.500, 1.00, 1.50 and 2.00-mL The fluorimeter readings from each solution are listed below. Calculate the concentration of PCP in the sample.
|
Spike VOl. (mL)
|
Spike amount (µg)
|
Spike conc.(µg/mL)
|
Readout
|
|
0.00
|
0.000
|
0.0000
|
3.26
|
|
0.500
|
1.000
|
0.02000
|
4.8
|
|
1.000
|
2.000
|
0.04000
|
6.41
|
|
1.500
|
3.000
|
0.06000
|
8.02
|
|
2.000
|
4.000
|
0.08000
|
9.56
|