Experiment: The Effect of Temperature
|
Time (min)
|
00C
|
23 0C (Baseline)
|
40 0C
|
60 0C
|
|
0
|
0
|
0
|
0
|
0
|
|
:30
|
.007
|
.017
|
0.11
|
.000
|
|
1:00
|
.021
|
.037
|
0.29
|
.001
|
|
1:30
|
.028
|
.062
|
0.50
|
.002
|
|
2:00
|
.042
|
.089
|
0.71
|
.002
|
|
2:30
|
.058
|
.117
|
.91
|
.003
|
|
3:00
|
.074
|
.145
|
.114
|
.003
|
|
3:30
|
.091
|
.172
|
.132
|
.004
|
|
4:00
|
.109
|
.197
|
.150
|
.004
|
|
4:30
|
.126
|
.222
|
.169
|
.004
|
|
5:00
|
.143
|
.247
|
.186
|
.004
|
5) Present Hypothesis
6) T Describe the effect of temperature on the reaction rate. Determine an optimal temperature for this enzyme.
7) Do your data support or reject your hypothesis? Explain how you know.
8) Explain how boiling (100°C) would affect this peroxidase enzyme.
Experiment: Absorbance results from effects of pH
|
Time (min)
|
PH =3
|
PH =5(Baseline)
|
pH =7
|
pH =9
|
|
0
|
0
|
0
|
0
|
0-
|
|
:30
|
-0.002
|
0.006
|
0.026
|
0.037
|
|
1:00
|
0.003
|
0.017
|
0.061
|
0.068
|
|
1:30
|
0.011
|
0.032
|
0.098
|
0.099
|
|
2:00
|
0.020
|
0.050
|
0.131
|
0.128
|
|
2:30
|
0.033
|
0.069
|
0.162
|
0.155
|
|
3:00
|
0.039
|
0.089
|
0.192
|
0.181
|
|
3:30
|
0.048
|
0.110
|
0.220
|
0.0206
|
|
4:00
|
0.057
|
0.129
|
0.248
|
0.0231
|
|
4:30
|
0.065
|
0.148
|
0.275
|
0.025
|
|
5:00
|
0.074
|
0.167
|
0.301
|
0.275
|
5. Present Hypothesis
6. Describe the effect of pH on the reaction rate. Determine an optimal pH for this enzyme.
7. Do your data support or reject your hypothesis? Explain how you know.
8. Explain how and why the optimal pH would differ for an enzyme found in Heliobacter pylori, a bacterium that lives in the human stomach.