Supposing you have isolated a set of conditional mutants in each enzyme of the pathway.
Call them 1, 2, 3, 4, 5, 6 and 7. The following feeding table is observed, in which growth is observed if the compound is added:
|
Mutant
|
PEP
|
3PG
|
G3P
|
F1,6DP
|
|
1
|
+
|
-
|
-
|
-
|
|
2
|
+
|
-
|
-
|
-
|
|
3
|
+
|
+
|
-
|
-
|
|
4
|
+
|
+
|
-
|
-
|
|
5
|
+
|
+
|
+
|
-
|
|
6
|
+
|
+
|
+
|
+
|
|
7
|
+
|
+
|
+
|
+
|
Order the mutants using these data and specify a possible enzyme that is temperature sensitive for each.