Problem:
Deascribe the following question:
Question 1: The isoelectric point (pI) of the hormone insulin is 6.5. Suggest a modification of the insulin Bchain that makes it more insoluble at physiological pH, without significantly affecting its binding to the Insulin Receptor (IR)
Question 2: Suggest a protein target for a drug thats effects would complement exogenous delivery of insulin in Type 2 Diabetes. Justify your answer.
Question 3: An insulininduced signaling assay has been developed with the use of cancer HeLa cells. Assess briefly the reliability of this assay