Question- the figure in appendix i gives an elution profile, Chemistry

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Question- the figure in appendix i gives an elution profile

Question- The figure in Appendix I gives an elution profile for the following proteins using Sephadex G-100 (Fractionation range 4KDa - 150 kDa):

0.9 KDa Amanitin

5 KDa Aprotinin

80 kDa Transferrin

135kDa Calmodulin

Part a) Give the Ve, in ml, for each protein.

Part b) Determine the VT and Vo for this column. If one or the other cannot be calculated, clearly state why.

Solve the above problem

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Chemistry: Question- the figure in appendix i gives an elution profile

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Chemistry: Question- the figure in appendix i gives an elution profile

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