Q1. There is a rainbow of GFP variants available. How is it probable to obtain this variety of fluorescing colours? Is it possible to obtain a "GFP variant" with the similar chromospheres as the original GFP? Explain the answer.
Q2. In the preparation of the esters in this experiment, the reaction product was extracted with 5 percent sodium bicarbonate solution (NaHCO3) in the isolation step. Why? What gas was evolved throughout the washing step?
Q3. Given an amount of sunlight that hits the plants on our planet, and ability of plants for rapid growth and reproduction, how come we are not all hip deep in dead plants?