Q1. The diploid number of the horse (Equus caballus) is 64 which include 36 acrocentric autosomes. The ass (Equus asinus) has 62 chromosomes including 22 acrocentric autosomes.
A) Predict the number of chromosomes that would be found in hybrid offspring (mule) produced by mating a male ass (jack) to a female horse (mare).
B) Why are mules frequently sterile (incapable of producing viable gametes)?
Q2. A plasmid digest with BamHI produced 2 kb and 4 kb fragments. Digest of a plasmid with HindIII produced 6 kb fragment. Double digest with both enzymes produced 1 kb, 2 kb and 3 kb fragments. Sketch a plasmid map.