Q. Acid Hydrolysis of Non-Reducing Sugar?
It involves the acid hydrolysis of non-reducing sugars to reducing sugars. It is also known as the process of inversion. Acid hydrolysis results in breakdown of sucrose into glucose and fructose. The process of inversion can be carried out in the following manner:
1) Place accurately 1 gm of honey solution in 250 ml of volumetric flask.
2) Dilute with about 150 ml of water.
3) Mix thoroughly contents of glass and make the volume to 250 ml.
4) Take 100 ml of this solution and add 6 ml of 0.04 N HCl.
5) Heat the solution to boiling.
6) Keep for ½ an hour.
7) Neutralize this inverted honey solution with 0.6N NaOH.