Question 1: What is the purpose of inverting inoculated plates during incubation?
Question 2: Define or explain:
i) Aseptic Technique
ii) Axenic Culture
iii) Complex or Nutrient media
iv) Anaerobic
v) Defined/Synthetic media
Question 3: What is the purpose of oil immersion with the microscope that you use in your class? What can't you view viruses with your microscope?
Question 4: What is the total magnification of your microscope if the ocular has 10X, the objective lens is 43X and the condenser lens is 20X.
Question 5: Why is it required to fix the bacterial smear on the slide, what is the advantage or disadvantage to "Heat fix" or "Air dry" bacterial smears. What may happen during staining if the smear is not fixed?
Question 6: A mixed smear of Staph. Epidermidis and Ecoli is Gram stained. Describe how the smear will stain under the following conditions:
i) Smears stained for 24-hour old culture
ii) Smears stained from 7-day old culture
Question 7: Why should controls is used with each experimental procedure?