Problem
The following DNA sequence has been identified and named 'gene X'. It is thought that this sequence codes for a single protein.
1. Design 5' and 3' PCR primers that will amplify ONLY the open reading frame of the genes. Give these sequences in a 5' to 3' orientation.
2. Include EcoR1 and NotI restriction enzyme recognition sites on the 5' ends of the 5' and 3' primers respectively.
3. Provide an accurate PCR program that will amplify the sequence.
4. Provide the amino acid sequence of the protein encoded by gene. Provide details of the theoretical molecular weight and isoelectric point of the protein.
5. Use your bioinformatics skills to provide details of the TM regions of the proteins.
6. Name the protein and provide a brief description of its role in health and disease. Remember to label figures appropriately and provide figure legends.