The hexapeptide N-acetyl-ala-arg-phosphoser-cys-met-glu has pKas of 1.6, 4.0, 4.2, 6.5, 8.1, and 12.1. (You will need to assign the pKas to particular groups.)
a. If this hexapeptide is hydrolyzed by trypsin, what products will result?
b. If these products are separated by DEAE-ion-exchange chromatography at pH 7.0, in what order will the products emerge from the column? Explain Why.