Procedure for Morphological Characteristics of Yeast Cells?
Now carry out the exercise following the steps enumerated herewith:
(1) Label the clean, non-greasy slide. Put few drops of water-iodine solution (Gram's Iodine-10ml, Distilled water - 30ml) on it.
(2) Suspend a loop full of yeast culture in the water-iodine solution and cover with the cover slip. Instead of water-iodine solution lactophenol - cotton blue stain can also be used.
(3) Observe the slide preparation under low and high power for the shape, size and budding or fission in the yeast. Draw a representative microscopic field.