Problem- A highly supercoiled DNA molecule is cleaved at one site by a restriction endonuclease, yielding linear DNA. Predict whether each of the following will increase, decrease or remain unchanged as a consequence of this treatment.
1. Second virial coefficient
2. Osmotic pressure at 0.1 mg/ml
3. Limit of pi/C as C --> 0
4. Diffusion coefficient
Please show how you obtained the answer