Problem
Researchers analyzing regulation of SIRT3 gene expression engineer a SIRT3 gene that is identical to the normal gene except that the normal SIRT3 gene promoter is replaced by the promoter for a gene encoding a protein typically found only in human nerve cells. The researchers introduce this gene into normal human stomach cells to determine how much SIRT3 mRNA AND protein will be expressed from the engineered SIRT3 gene in the stomach cells. Predict the results of this experiment. The researchers claim that metabolic activity increases in cancerous cells. Provide reasoning based on the data provided to justify the claim. Based on the information provided, predict the cause of a decrease in CO2 production by the three groups of cancer cells (SC-1, SC+plasmid, and SC+RNA) treated with the compound that blocks the electron transport chain compared with the same groups of cells not treated with the compound.