Practice problems for stock solutions General Microbiology
1. You need to make 400ml of a 75x stock solution of TAE buffer. The final concentration (or working solution) of TAE is 40mM Tris acetate (M.W 121.14g/mol) and 2mM EDTA (M.W 372.24g/mol). How would you accomplish this?
2. You need 1.5L of 1x TAE buffer from your 75x stock solution of TAE buffer. How would you accomplish this?
3. You need to make 40ml of a 1.2% agarose gel. How would you accomplish this?