Polymerase chain reaction already has extremely widespread applications and new uses are being devised on a regular basis. Some and definitely not all of the applications are given as follows:
? PCR can amplify a one DNA molecule from a complex mixture, largely preventing the requirement to use DNA cloning to arrange that molecule. Different of the methods can similarly amplify a particular single RNA molecule from a complex combination.
? DNA sequencing has been broadly simplified using Polymerase chain reaction, and this application is now general.
? Through using suitable primers it should be possible to use PCR to make point mutations, deletions and insertions of target DNA that greatly facilitates the analysis of gene expression and function.
? Polymerase chain reaction is exquisitely sensitive and can amplify vanishingly small amounts of DNA. Thus, using appropriate primers very little amounts of specified viruses and bacteria can be detected in tissues and making PCR invaluable for medical diagnosis.